Leptomycin B
Cat. Number: FA-00388
Size: 1g, 5g, 10g, Custom size
Description
Leptomycin B is an anti-fungal antibiotic, anti-tumor cytotoxin that inhibits CRM1-dependent, NES-dependent nucleo-cytoplasmic translocation (nuclear export inhibitor). NES containing proteins include HIV-1 REV; actin, c-Abl, cyclin B1, MDM2/p53, MPF, PKA and MEK.
Product Details
CAS Number
87081-35-4
Source
Streptomyces sp.
Linear Formula
C33H48O6
Appearance
Solution
Molecular Weight
540.73
Storage Temp.
-20 °C (protect from light)
Antibiotic Activity Spectrum
fungi
Mode of Action
protein synthesis/interferes
SMILES
CCC (\C=C\[C@@H]1OC (=O)C=C[C@@H]1C)=C\[C@H] (C)C\C=C\C (C)=C\[C@@H] (C)C (=O)[C@@H] (C)[C@H] (O)[C@@H] (C)C\C (C)=C\C (O)=O
InChI
1S/C33H48O6/c1-9-28 (14-15-29-24 (5)13-16-31 (36)39-29)19-22 (3)12-10-11-21 (2)17-25 (6)32 (37)27 (8)33 (38)26 (7)18-23 (4)20-30 (34)35/h10-11,13-17,19-20,22,24-27,29,33,38H,9,12,18H2,1-8H3, (H,34,35)/b11-10+,15-14+,21-17+,23-20+,28-19-/t22-,24+,25-,26+,27-,29+,33-/m1/s1
InChI key
YACHGFWEQXFSBS-XYERBDPFSA-N
Handling Precaution
Leptomycin B is an unsaturated, branched-chain fatty acid, and is an important tool in the study of nuclear export. Leptomycin B is a specific inhibitor of proteins containing nuclear export signal. Leptomycin B inhibits nucleo-cytoplasmic translocation of molecules such as the HIV-1 Rev protein and Rev-dependent export of mRNA. The addition of very small amounts to fibroblasts causes accumulation of MEK in the nucleus. Other proteins that are influenced by leptomycin B are actin, c-Abl, cyclin B1, MDM2/p53, IκB, MPF, and PKA. The suggested inhibition mechanism involves the direct binding of leptomycin B to CRM1, which blocks the binding of CRM1 to proteins containing the nuclear export signal, via the interaction with cysteine residue in CRM1 control conserved region.
For Research Use Only!
